Alexander PA, He Y, Chen Y, Orban J, Bryan PN. The design and characterization of two proteins with 88% sequence identity but different structure and function. Proc Natl Acad Sci U S A. 2007 Jul 3; [Epub ahead of print] PMID: 17609385 [PubMed - as supplied by publisher]

Sari N, Ruan B, Fisher KE, Alexander PA, Orban J, Bryan PN. Hydrogen-deuterium exchange in free and prodomain-complexed subtilisin. Biochemistry. 2007 Jan 23;46(3):652-8. PMID: 17223687 [PubMed - indexed for MEDLINE]

Fisher KE, Ruan B, Alexander PA, Wang L, Bryan PN. Mechanism of the kinetically-controlled folding reaction of subtilisin. Biochemistry. 2007 Jan 23;46(3):640-51. PMID: 17223686 [PubMed - indexed for MEDLINE]

He Y, Rozak DA, Sari N, Chen Y, Bryan P, Orban J. Structure, dynamics, and stability variation in bacterial albumin binding modules: implications for species specificity. Biochemistry. 2006 Aug 22;45(33):10102-9. PMID: 16906768 [PubMed - indexed for MEDLINE]

Rozak DA, Alexander PA, He Y, Chen Y, Orban J, Bryan PN. Using offset recombinant polymerase chain reaction to identify functional determinants in a common family of bacterial albumin binding domains. Biochemistry. 2006 Mar 14;45(10):3263-71. PMID: 16519521 [PubMed - indexed for MEDLINE]

Rozak DA, Orban J, Bryan PN. G148-GA3: a streptococcal virulence module with atypical thermodynamics of folding optimally binds human serum albumin at physiological temperatures. Biochim Biophys Acta. 2005 Dec 1;1753(2):226-33. Epub 2005 Oct 26. PMID: 16290081 [PubMed - indexed for MEDLINE]

He Y, Yeh DC, Alexander P, Bryan PN, Orban J. Solution NMR structures of IgG binding domains with artificially evolved high levels of sequence identity but different folds. Biochemistry. 2005 Nov 1;44(43):14055-61. PMID: 16245921 [PubMed - indexed for MEDLINE]

Alexander PA, Rozak DA, Orban J, Bryan PN. Directed evolution of highly homologous proteins with different folds by phage display: implications for the protein folding code. Biochemistry. 2005 Nov 1;44(43):14045-54. PMID: 16245920 [PubMed - indexed for MEDLINE]

Abdulaev NG, Zhang C, Dinh A, Ngo T, Bryan PN, Brabazon DM, Marino JP, Ridge KD. Bacterial expression and one-step purification of an isotope-labeled heterotrimeric G-protein alpha-subunit. J Biomol NMR. 2005 May;32(1):31-40. PMID: 16041481 [PubMed - indexed for MEDLINE]

Rozak DA, Bryan PN. Offset recombinant PCR: a simple but effective method for shuffling compact heterologous domains. Nucleic Acids Res. 2005 May 18;33(9):e82. PMID: 15901853 [PubMed - indexed for MEDLINE]

Strausberg SL, Ruan B, Fisher KE, Alexander PA, Bryan PN. Directed coevolution of stability and catalytic activity in calcium-freesubtilisin. Biochemistry. 2005 Mar 8;44(9):3272-9. PMID: 15736937 [PubMed - indexed for MEDLINE]

Ruan B, Fisher KE, Alexander PA, Doroshko V, Bryan PN. Engineering subtilisin into a fluoride-triggered processing protease useful for one-step protein purification.
Biochemistry. 2004 Nov 23;43(46):14539-46. PMID: 15544324 [PubMed - indexed for MEDLINE]

Bryan PN. Prodomains and protein folding catalysis. Chem Rev. 2002 Dec;102(12):4805-16. Review. No abstract available. PMID: 12475207 [PubMed - indexed for MEDLINE]

Tangrea MA, Bryan PN, Sari N, Orban J. Solution structure of the pro-hormone convertase 1 pro-domain from Mus musculus. J Mol Biol. 2002 Jul 19;320(4):801-12. PMID: 12095256 [PubMed - indexed for MEDLINE]

Alexander PA, Ruan B, Strausberg SL, Bryan PN. Stabilizing mutations and calcium-dependent stability of subtilisin. Biochemistry. 2001 Sep 4;40(35):10640-4.
PMID: 11524008 [PubMed - indexed for MEDLINE]

Alexander PA, Ruan B, Bryan PN. Cation-dependent stability of subtilisin.
Biochemistry. 2001 Sep 4;40(35):10634-9. PMID: 11524007 [PubMed - indexed for MEDLINE]

Tangrea MA, Alexander P, Bryan PN, Eisenstein E, Toedt J, Orban J. Stability and global fold of the mouse prohormone convertase 1 pro-domain. Biochemistry. 2001 May 8;40(18):5488-95. PMID: 11331013 [PubMed - indexed for MEDLINE]

Bryan PN. Protein engineering of subtilisin. Biochim Biophys Acta. 2000 Dec 29;1543(2):203-222. Review. PMID: 11150607 [PubMed - indexed for MEDLINE]

Sari N, Alexander P, Bryan PN, Orban J. Structure and dynamics of an acid-denatured protein G mutant. Biochemistry. 2000 Feb 8;39(5):965-77. PMID: 10653640 [PubMed - indexed for MEDLINE]

Ruan B, Hoskins J, Bryan PN. Rapid folding of calcium-free subtilisin by a stabilized pro-domain mutant. Biochemistry. 1999 Jun 29;38(26):8562-71. PMID: 10387104 [PubMed - indexed for MEDLINE]

Ruan B, Hoskins J, Wang L, Bryan PN. Stabilizing the subtilisin BPN' pro-domain by phage display selection: how restrictive is the amino acid code for maximum protein stability? Protein Sci. 1998 Nov;7(11):2345-53. PMID: 9828000 [PubMed - indexed for MEDLINE]

Almog O, Gallagher T, Tordova M, Hoskins J, Bryan P, Gilliland GL. Crystal structure of calcium-independent subtilisin BPN' with restored thermal stability folded without the prodomain. Proteins. 1998 Apr 1;31(1):21-32. PMID: 9552156 [PubMed - indexed for MEDLINE]

Wang L, Ruan B, Ruvinov S, Bryan PN. Engineering the independent folding of the subtilisin BPN' pro-domain: correlation of pro-domain stability with the rate of subtilisin folding. Biochemistry. 1998 Mar 3;37(9):3165-71. PMID: 9485470 [PubMed - indexed for MEDLINE]

Ruvinov S, Wang L, Ruan B, Almog O, Gilliland GL, Eisenstein E, Bryan PN.
Engineering the independent folding of the subtilisin BPN' prodomain: analysis
of two-state folding versus protein stability. Biochemistry. 1997 Aug 26;36(34):10414-21. PMID: 9265621 [PubMed - indexed for MEDLINE]

Khare D, Alexander P, Antosiewicz J, Bryan P, Gilson M, Orban J. pKa measurements from nuclear magnetic resonance for the B1 and B2 immunoglobulin G-binding domains of protein G: comparison with calculated values for nuclear magnetic resonance and X-ray structures. Biochemistry. 1997 Mar 25;36(12):3580-9. PMID: 9132009 [PubMed - indexed for MEDLINE]

Gilliland GL, Gallagher DT, Alexander P, Bryan P. Crystal structure analysis of subtilisin BPN' mutants engineered for studying thermal stability. Adv Exp Med Biol. 1996;379:159-69. PMID: 8796321 [PubMed - indexed for MEDLINE]

Wang L, Ruvinov S, Strausberg S, Gallagher DT, Gilliland G, Bryan PN. Prodomain mutations at the subtilisin interface: correlation of binding energy and the rate of catalyzed folding. Biochemistry. 1995 Nov 28;34(47):15415-20. PMID: 7492541 [PubMed - indexed for MEDLINE]

Orban J, Alexander P, Bryan P, Khare D. Assessment of stability differences in the protein G B1 and B2 domains from hydrogen-deuterium exchange: comparison with calorimetric data. Biochemistry. 1995 Nov 21;34(46):15291-300. PMID: 7578145 [PubMed - indexed for MEDLINE]

Gallagher T, Gilliland G, Wang L, Bryan P. The prosegment-subtilisin BPN' complex: crystal structure of a specific 'foldase'. Structure. 1995 Sep 15;3(9):907-14. PMID: 8535784 [PubMed - indexed for MEDLINE]

Bryan P, Wang L, Hoskins J, Ruvinov S, Strausberg S, Alexander P, Almog O,
Gilliland G, Gallagher T. Catalysis of a protein folding reaction: mechanistic implications of the 2.0 A structure of the subtilisin-prodomain complex.
Biochemistry. 1995 Aug 15;34(32):10310-8. PMID: 7640287 [PubMed - indexed for MEDLINE]

Strausberg SL, Alexander PA, Gallagher DT, Gilliland GL, Barnett BL, Bryan PN.
Directed evolution of a subtilisin with calcium-independent stability. Biotechnology (N Y). 1995 Jul;13(7):669-73. PMID: 9634803 [PubMed - indexed for MEDLINE]

Bryan PN. Site-directed mutagenesis to study protein folding and stability. Methods Mol Biol. 1995;40:271-89. Review. No abstract available. PMID: 7633526 [PubMed - indexed for MEDLINE]

Orban J, Alexander P, Bryan P. Hydrogen-deuterium exchange in the free and immunoglobulin G-bound protein G B-domain. Biochemistry. 1994 May 17;33(19):5702-10. PMID: 8180196 [PubMed - indexed for MEDLINE]

Gallagher T, Alexander P, Bryan P, Gilliland GL. Two crystal structures of the B1 immunoglobulin-binding domain of streptococcal protein G and comparison with NMR. Biochemistry. 1994 Apr 19;33(15):4721-9. PMID: 8161530 [PubMed - indexed for MEDLINE]

Strausberg S, Alexander P, Wang L, Gallagher T, Gilliland G, Bryan P. An engineered disulfide cross-link accelerates the refolding rate of calcium-free subtilisin by 850-fold. Biochemistry. 1993 Oct 5;32(39):10371-7. PMID: 8399180 [PubMed - indexed for MEDLINE]

Strausberg S, Alexander P, Wang L, Schwarz F, Bryan P. Catalysis of a protein folding reaction: thermodynamic and kinetic analysis of subtilisin BPN' interactions with its propeptide fragment. Biochemistry. 1993 Aug 17;32(32):8112-9. PMID: 8347611 [PubMed - indexed for MEDLINE]

Gallagher T, Bryan P, Gilliland GL. Calcium-independent subtilisin by design. Proteins. 1993 Jun;16(2):205-13. PMID: 8332608 [PubMed - indexed for MEDLINE]

Alexander P, Orban J, Bryan P. Kinetic analysis of folding and unfolding the 56 amino acid IgG-binding domain of streptococcal protein G. Biochemistry. 1992 Aug 18;31(32):7243-8. PMID: 1510916 [PubMed - indexed for MEDLINE]

Bryan P, Alexander P, Strausberg S, Schwarz F, Lan W, Gilliland G, Gallagher DT.
Energetics of folding subtilisin BPN'. Biochemistry. 1992 Jun 2;31(21):4937-45. PMID: 1599918 [PubMed - indexed for MEDLINE]

Alexander P, Fahnestock S, Lee T, Orban J, Bryan P. Thermodynamic analysis of the folding of the streptococcal protein G IgG-binding domains B1 and B2: why small proteins tend to have high denaturation temperatures. Biochemistry. 1992 Apr 14;31(14):3597-603. PMID: 1567818 [PubMed - indexed for MEDLINE]

Orban J, Alexander P, Bryan P. Sequence-specific 1H NMR assignments and secondary structure of the streptococcal protein G B2-domain. Biochemistry. 1992 Apr 14;31(14):3604-11. PMID: 1314644 [PubMed - indexed for MEDLINE]