Research Overview
Age-Related Diseases
Changes in the quantitative pattern of
proteins associated with aging processes are providing insight into
disease pathologies and may help in early diagnosis ... Read More
Multiple Reaction Monitoring (MRM)
MRM approach has been developed to
measure specific peptides in complex biological mixtures. Proteases
are first used to.... Read More
Hydrogen-Deuterium Exchange Mass
Spectrometry (H-D exchange MS)
Amide H-D exchange MS is a sensitive
technique that can be used to study the changes in protein structure
and dynamics due to protein-protein interaction, ligand... Read More
Relative Quantification with Stable
Isotope Labeling
To estimate the relative amount of a
specific protein in two separate samples (control versus experiment),
the proteins in control sample are alkylated with light... Read More
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Research Description
Research Area: Genomics and
Proteomics
Research Specialty: Proteomics
of Aging
The lab is focused on the mass
spectrometry-based quantitative techniques to assess age-related
proteomic changes in brain and retina.
Current scientific interests include:
(1) Multiple Reaction Monitoring
Measurements for proteins involved in the generation, disposal, and
function of oxysterols in the senescent retinal pigmented epithelium.
(2) Hydrogen-Deuterium Exchange Mass
Spectrometry for analysis of structural changes in cytochrome P450s
during interactions with substrates, inhibitors, and protein
partners.
(3) Quantitative protein profiling of
age-related diseases of human brain and retina.
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Representative Publications
Mast, N., Liao, W.L., Pikuleva, I.A.,
and Turko, I.V.: Combined use of mass spectrometry and heterologous
expression for identification of membrane-interacting peptides in
cytochrome P450 46A1 and NADPH-cytochrome P450 oxidoreductase (2009)
Arch. Biochem. Biophys., in press.
Pikuleva, I.A., Mast, N., Liao, W.L.,
and Turko, I.V.: Studies of membrane topology of mitochondrial
cholesterol hydroxylases CYPs 27A1 and 11A1 (2008) Lipids 43,
1127-1132.
Liao, W.L., and Turko, I. V.: Strategy
combining separation of isotope-labeled unfolded proteins and
matrix-assisted laser desorption/ionization mass spectrometry
analysis enables quantification of a wide range of serum proteins
(2008) Anal. Biochem. 377, 55-61.
Turko, I. V., and Sechi, S.: Acrylamide
- a cysteine alkylating reagent for quantitative proteomics (2007)
Methods Mol. Biol. 359, 1-16.
Turko, I. V., and Murad, F.: Mapping
sites of tyrosine nitration by matrix-assisted laser
desorption/ionization mass spectrometry (2005) Methods Enzymol.
396, 266-275.
Complete Listing...
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